Department of Microbiology, Ganesh Scientific Research Foundation (GSRF), Kirti Nagar, New Delhi-110015, India
*Address for Corresponding author
Department of Microbiology, Ganesh Scientific Research Foundation (GSRF),
Kirti Nagar, New Delhi-110015, India
Background: Agro-wastes are potential alternative raw materials for the production of several value-added metabolites. Solid-state fermentation is used for microbial production of bioactive compounds especially enzymes, due to its advantages, such as usage of low-cost raw materials, less effluent generation, and production of biocatalysts with unique catalytic properties. Objective: This study mainly focuses on the optimization of process parameters for the synthesis of asparaginase in solid-state fermentation by Fusarium oxysporum (NCIM 1008) in a production medium, containing corn husk as substrate. Methods: Production of asparaginase in solid-state fermentation by Fusarium oxysporum (NCIM 1008) was investigated. The impact of initial moisture content, fermentation time, initial pH, and fermentation temperature, effect of additional carbon and nitrogen sources, on the production of enzyme was studied. Results: Maximal enzyme activity (27.10 U/gds) with corn husk was achieved with following optimised conditions: initial moisture content 65% (v/w), initial pH 6.0, supplemented with xylose 0.5% (w/w), urea 1% (w/w) incubated at 28°C for 72 h. Conclusion: Even though corn husk is high in cellulose content, it supported better microbial cell growth with good enzyme productivity. After optimization, asparaginase activity obtained is comparable with the activity of asparaginases, synthesised from other fungal strains. The process optimization and inexpensive substrate usage makes solid-state fermentation more economical for the production of asparaginase.
Keywords: Asparaginase, Fusarium oxysporum, Solid-state fermentation, Corn husk