Research Articles

2019  |  Vol: 5(4)  |  Issue: 4 (July- August)  |  https://doi.org/10.31024/ajpp.2019.5.4.22
Diosgenin decrease proliferation of human lung cancer cells by promoting cholesterol efflux via up-regulation of LXR-α and its target ABCA1 expression

Nithya Ganesan, Antony Joseph Velanganni Arockiam*

Molecular Oncology Lab, Department of Biochemistry, Bharathidasan University, Tiruchirapalli-620024, Tamil Nadu, India

*Address for Corresponding Author        

Dr. A. Antony Joseph Velanganni,

Molecular Oncology Lab, Department of Biochemistry, Bharathidasan University, Tiruchirapalli-620024, Tamil Nadu, India

Abstract

Background: Several reports are available on the antitumor effects of diosgenin in various cancer cells. However, the effect of diosgenin on LXR-α and its target ABCA1 has not been studied in lung cancer cells. Objective: To inspect the effect of diosgenin reduce cell proliferation in lung cancer cell line by the induction of LXR-α and ABCA1 in lung cancer cell line. Materials and Methods: Cell viability was determined by MTT assay, Cells proliferation was determined by Wound healing/scratch assay and mRNA and proteins expression were examined by Semi-Quantitative RT- PCR. Results: Diosgenin inhibits A549 cell viability in a dose dependent manner and was proved by MTT assay. Diosgenin inhibits proliferation of A549 cell wound healing/scratch assay. Diosgenin (45 μM) induces mitochondrial mediated apoptosis by the activation of caspase-3, up-regulation of LXR-α, ABCA1, Bax, caspase-3and down-regulation of Bcl-2. Conclusion: Diosgenin reduce proliferation in A549 cells by promoting cholesterol efflux via up-reguatinof LXR-α and ABCA1 expression.

Keyword: ATP-binding cassette transporter A1.Diosgenin.Lung cancer.Liver x receptor-α

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