Research Articles

2019  |  Vol: 5(1)  |  Issue: 1(January-February)  |  https://doi.org/10.31024/ajpp.2019.5.1.9
HPLC analysis of water soluble vitamin B in Psidium guava leaves

Raja Suja Pandian*, M. Jayalakshmi

Post Graduate and Research Department of Biochemistry,

Rabiammal Ahamed Maiden College for Women, Thiruvarur, Tamilnadu, India.

*Address for Corresponding Author

Dr. R. Suja Pandian

Assistant professor

Post Graduate and Research Department of Biochemistry

Rabiammal Ahamed Maiden College for Women, Thiruvarur, Tamilnadu, India.

Abstract

Objective: The main objective of the present work is to analyse the water soluble B vitamins present in Psidium guava leaves using HPLC. Materials and Methods: The fresh leaves of Psidium guajava. L collected were shade dried, grinded to fine powder and kept in dry conditions .The plant powder was mixed with water and stirred continuously until it was reduced to one third and filtered. The filtrate was evaporated to dryness. Paste form of the extract obtained was subjected to analysis using HPLC. Results: Under the described experimental conditions, the values of retention times were: 9.067min for Niacin, 12.567min for Pyridoxine, 23.358min for Thiamine and 25.867min for Riboflavin. Conclusion: It is concluded that Psidium guajava leaves are very much important for their medicinal as well as nutritional value. The leaves provide opportunities to develop as medicines, value added products and dietary supplements which possess potential health benefits. The leaves were rich sources of valuable bioactive compounds and can be explored as nutraceuticals.

Keywords: Psidium guava leaves, HPLC, B vitamins

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