Dileep Kumar H. V.a,b, Shivanandappa T. a,b*
aNeurobiology Lab, Department of Zoology, University of Mysore, Manasagangotri, Mysore-570006, Karnataka, India
bDepartment of Food Protectants and Infestation Control, CSIR-Central Food Technological Research Institute, Mysore-570020, Karnataka, India
*Address for Corresponding Author
Department of Zoology, University of Mysore, Manasagangotri, Mysore– 570 006 Karnataka, India
Objective: 4-hydroxyisophthalic acid (4-HIPA) is a novel bioactive molecule isolated in our laboratory from the edible roots of Decalepis hamiltonii. Earlier we have shown free radical scavenging activity of 4-HIPA in vitro and cytoprotective action. Here we report in vivo neuroprotective potential of 4-HIPA against ethanol-induced oxidative stress in the brain of laboratory rat. Materials and Methods: Rats were treated with ethanol (5g/kg bw) in single and multiple doses with/without pre-treatment with 4-HIPA (200mg/kg bw). Results: Ethanol-induced oxidative stress in both single and multiple dose treatment which showed regional differences. Oxidative stress in the cerebral cortex and cerebellum being higher in the latter as indicated by the increased reactive oxygen species, lipid peroxidation, protein carbonyls and glutathione depletion. Pre-treatment with 4-HIPA significantly restored the alterations induced by ethanol. 4-HIPA protected against ethanol-induced oxidative stress by boosting the antioxidant enzymes, superoxide dismutase, catalase, glutathione peroxidase, glutathione-S-transferase and glutathione reductase. Conclusion: 4-HIPA is a potential neuroprotective molecule of natural origin and shows the promise natural bioactive compound derived from edible source against alcohol toxicity. Therefore, 4-HIPA has health implications as a nutraceutical against alcohol abuse.
Keywords: Decalepis hamiltonii, 4-HIPA, neuroprotection, ethanol, GSH, ROS, antioxidant enzymes