Research Articles

2018  |  Vol: 4(6)  |  Issue: 6 (November-December)  |  https://doi.org/10.31024/ajpp.2018.4.6.7
Anticancer activity of Asparagus racemosus root extracts in non-small cell lung cancer A549 cells

Debaashish Biswasa, Manisha Mathurc, Hemant Malhotrab, Shipra Bhargavaa, Bharti Malhotraa*

aDepartment of Microbiology, SMS Medical College, Jaipur (Rajasthan) India

bDivision of Medical Oncology, Department of Medicine, SMS Medical College, Jaipur (Rajasthan) India

cGovernment G.D. Girls College, Alwar (Rajasthan) India

*Address of Corresponding Author:

Dr. Bharti Malhotra

Senior Professor, Department of Microbiology and Immunology,

S.M.S. Medical College, J.L.N. Marg, Near Albert Hall Museum,

Ram Niwas Garden, Jaipur, Rajasthan- 302004 India

Abstract

Background: Lung cancer is the leading cause of cancer-related mortality worldwide. Non-small-cell lung cancer (NSCLC) accounts for 85% of all lung cancer cases. Recently emerged molecular targeted therapies have improved the treatment of lung cancer patients. But still, the problems like drug resistance, cancer recurrence and drug side effects persist with the current therapies. Therefore, the development of new chemotherapeutic agents has become an urgent need for patients to benefit and better survival. Asparagus racemosus is an important medicinal plant exhibiting the anti-cancer activity against various cancers; however, its activity against lung cancer remains to be addressed. Objective: In this study, we have assessed the anti-cancer activity of Asparagus racemosus root extracts in human lung adenocarcinoma cell line A549. Material and methods: The cytotoxic activity of the chloroform: methanol and methanol extract was determined by using MTT (3-[4, 5-dimethyl-2-thiazolyl]-2,5 diphenyltetrazolium bromide) assay, change in morphology by morphological analysis and reduction in migration was done by cell migration assay. Results: Significant cytotoxic effect of methanol extract (IC50 100.5 µg/ml) in comparison to chloroform: methanol extract (IC50  136.5 µg/ml) was observed. Treatment with the extracts changed the morphology of the cells, as cells become round in shape and migration reduced after treatment with root extracts. Conclusions: This study showed that Asparagus racemosus root extracts can cause cytotoxic effects, change the morphology and induce growth inhibition in A549 cells. Therefore, it can be developed as a plant-derived drug to treat NSCLC patients in the future. 

Keywords: Non-small-cell lung cancer, MTT, Asparagus racemosus, lung adenocarcinoma

Manuscript Management System
Submit Article Subscribe Most Popular Articles Join as Reviewer Email Alerts Open Access
Our Another Journal
Another Journal