Research Articles

2019  |  Vol: 5(6)  |  Issue: 6 (November-December)  |  https://doi.org/10.31024/ajpp.2019.5.6.16
Phytochemical, antibacterial, antioxidant and wound healing properties of Aloe inermis latex extract obtained from Yemen

Bushra Abdulkarim Moharrama,*, Hassan M. Al-Mahbashib, Tareq Al-MaqtariaRiyadh Saif-AlicAmin A Al-Doaissd,e

aDepartment of Pharmacognosy, Faculty of Pharmacy, Sana’a University, Sana’a, Yemen.

bDepartment of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Sana`a University, Sanaa, Yemen.

aDepartment of Pharmacognosy, Faculty of Pharmacy, Sana’a University, Sana’a, Yemen.

cDepartment of Biochemistry and Molecular Biology, Faculty of Medicine, Sana’a University, Sana’a. Yemen.

*Address for Corresponding Author

Bushra Abdulkarim Moharram (PhD)

Assistant Professor of Pharmacognocy

Faculty of Pharmacy, Sana’a University, Yemen

Abstract

Objective: To determine the phytochemical content and to evaluate the antibacterial, antioxidant, and wound healing activities of the methanol extracts of A. inermis. Material and Methods: The methanol extracts of the latex, gel and green skin of A. inermis were screened for their phytochemical content. The antibacterial activity of the extracts was investigated using disc diffusion and broth microdilution assays and the antioxidant activity was evaluated using the DPPH free radical scavenging assay. The wound healing activity of the latex extract was evaluated via histological analyses and area measurements of healing wounds in rats. Results: The methanol extracts of A. inermis parts contained carbohydrates, steroids, phenolic compounds, tannins, anthraglycosides, anthrons and bitter compounds. The latex extract of A. inermis exerted a strong antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa with inhibition zones of 18 and 19 mm, respectively, with MIC values of 1.25 mg/ml and MBCs of 2.50 mg/ml against both microorganisms. The latex and green skin extracts exhibited the highest antioxidant activity on DPPH assay with IC50 values of 55.9 and 67.4µg/ml, respectively. Topical application of the latex extract significantly accelerated the rate of wound healing and was associated with less scarring. The newly-formed granulation tissue of healing wounds contained less inflammatory cells and more fibroblasts compared to wounds treated with the vehicle. Conclusion: The latex extract of A. inermis is a potential source of bioactive compounds that may be exploited in the treatment of bacterial infections and to promote wound healing. 

Keyword: Aloe inermis, antibacterial, antioxidant, latex extract, phytochemical, wound healing

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