Research Articles

2019  |  Vol: 5(2)  |  Issue: 2(March-April)  |  https://doi.org/10.31024/ajpp.2019.5.2.24
Subtractive genomics approach in identifying polysacharide biosynthesis protein as novel drug target against Eubacterium nodatum

Shilpa S. Shiragannavar, Arun K. Shettar, Shivakumar B. Madagi, Sunanda Sarawad1*

Department of Studies in Bioinformatics and Biotechnology, Akkamahadevi Women’s University Vijayapura-586108, Karnataka, India

*Address for Corresponding Author                                    

Ms. Sunanda Sarawad

Faculty of Science

Dept of Bioinformatics & Biotechnology,

Akkamahadevi Women’s University, Vijayapura-586108, Karnataka, India

Abstract

Objective: The present investigation was carried out to find the therapeutic drug targets against Eubacterium nodatumMaterials and Methods: Based on the CD-HITS 1627 proteins were selected among the total proteome count of 1690 proteins. BLASTP were used for sequence analysis which revealed around 1498 non homologues proteins in human genome. Database of Essential Gene (DEG) was used to study the high stringency analysis of the non homologues/remaining proteins which revealed around 807 essential proteins of Eubacterium nodatumResults: Metabolic pathway analysis of human host and pathogen was performed by KEGG server. The KEGG results sorted around 132 proteins among selected 807 proteins. Further among the132 human non –homologues proteins 20 unique non homologues essential proteins were considered through Psortb, Cello and SOSUI which shows that, the identified drug targets are exposed to have high potential for designing novel drug against Eubacterium nodatum. The Jpred and Phyre2 server were used to identify 2D and 3D structures of 16 membrane associated proteins and validation was done with RAMPAGE and Procheck. Further study investigated around 200 selective drugs using the drug bank, PubChem databases which revealed Cinnamoyl echinadiol as a single potent drug according to the FDA approved drug bank database with diverse ADMET, virtual screening and drug-likeliness property. Conclusion: Molecular docking analysis shows that, Polysaccharide biosynthesis protein as a novel drug target with Cinnamoyl echinadiol drug against pathogen Eubacterium nodatum.

KeywordsEubacterium nodatum, BLASTP, DEG, KEGG, Cinnamoyl echinadiol, Polysacharide biosynthesis protein

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