Research Articles

2021  |  Vol: 7(1)  |  Issue: 1(January-February)  |  https://doi.org/10.31024/ajpp.2021.7.1.3
In vitro antioxidative and cytotoxic effect of protein fraction from Tabernaemontana divaricata leaves against DLA cells

M. Srilatha1*, K. Lavanya2, Subha T.1

1Department of Biotechnology, Sona College of Arts and Science, Salem - 636 005, Tamilnadu, India

2Department of Biochemistry, PSG College of Arts & Science, Coimbatore- 641014 Tamil Nadu India

*Address for Corresponding Authors

M. Srilatha

Department of Biotechnology, Sona College of Arts and Science, Salem - 636 005, Tamilnadu, India

 

Abstract

Background: The rising burden of cancer worldwide calls for an alternative treatment solution. Herbal medicine provides a very feasible alternative to western medicine against cancer. Objectives: The present study assessed the in vitro antioxidative potential and cytotoxicity of Tabernaemontana divaricata leaves protein fraction. Materials and Methods: The antioxidant activity of protein fraction of T. divaricata was performed by several antioxidative assays including 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay, ABTS, superoxide radical (SO) scavenging assay and Hydrogen peroxide (H2O2) with standard protocol, followed by Cytotoxicity test with tryphan blue assay. Results: From the results, T.divaricata has been found to have the significant antioxidant activity in a dose-dependent manner and IC50 value was 59.1μg/ml for DPPH, 48 µg/ml for ABTS, 60 µg/ml for SO and 38.5 µg/ml for H2O2. Further, the cytotoxicity analysis was determined against Dalton Lymphoma Ascites (DLA) cell line and the IC50 value was found to be 62μg/ml for protein fraction of T.divaricata. Conclusion: Hence, the current study attests that T. divaricata is a fine source of natural antioxidants with anticancer agents and can be used in pharmaceutical preparations for the treatment of diseases induced by oxidative stress.

KeywordsT. divaricata, Antioxidants, Dalton Lymphoma ascites, Trypan blue assay

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