Research Articles

2019  |  Vol: 5(1)  |  Issue: 1(January-February)  |  https://doi.org/10.31024/ajpp.2019.5.1.8
Chemoprevention of breast cancer by Psidium guajava Linn.

Prachi D. KariaaLaxmi A. Patilb, Mitul S. Vakanic, Gaurav M. Chauhanc, Sarita S. Guptac, S. P. Rathodd, Kirti V. Patelb*

aITM School of Pharmacy, Dhanora Tank Road, Paldi Village, Halol Highway, Near Jarod, Vadodara, Gujarat, India

bFaculty of Pharmacy, The Maharaja Sayajirao University of Baroda,Vadodara, Gujarat, India

cDepartment of Biochemistry, Faculty of Science, The Maharaja Sayajirao University of Baroda, Vadodara, Gujarat, India

dParul Institute of Pharmacy and Research, Vadodara, Gujarat, India

*Address for Correspondence

Dr. Kirti V. Patel

Associate Professor

Faculty of Pharmacy,

The Maharaja Sayajirao University of Baroda, Vadodara – 390001, Gujarat, India

Abstract

Objective: The present study was performed to investigate the chemo-preventive potential of Pulp of Psidium guajava (POPG) in breast cancer and elucidating it’s mechanism of action by assessing its effect on key processes like apoptosis, angiogenesis and metastasis. Materials and Methods: The cytotoxicity assay of POPG was performed on MCF-7(ER+), MDA-MB-231(Triple negative) and MDA-MB-453 (HER2+) human breast cancer cell lines. Assessment of anticancer potential of POPG was done through measurement of growth rate, feed consumption efficiency, tumor parameters, estrogen and progesterone expressions & nucleic acid content in in-vivo study. The mechanism for anticancer potential was screened by in-vitro studies involving Migration assay (metastasis), Annexin V- FITC assay (apoptosis) and Chick Chorioallantoic Membrane assay (angiogenesis). Statistical analysis was done by ANOVA followed by bonferroni’s post-hoc test. Results: The IC50 value of POPG on MCF-7 cells was significantly less than other two cell lines, indicating POPG to be more potent inhibitor of ER+ cells in-vitro. Confirmatory results were obtained in MNU induced mammary carcinoma. POPG attenuated tumor parameters, expression of estrogen and progesterone receptor, nucleic acid content and increased latency period. POPG prevented MCF-7 cell migration (66.67%) suggesting inhibition of metastasis. POPG significantly increased apoptotic rate, especially late apoptotic population (6.1%). The mean zone of inhibition in CAM assay was found to be 1.13±0.33 implying inhibition of neovascularization. Conclusion: POPG thus depicts chemoprevention of breast cancer and this could be attributed to its ability to induce apoptosis, curtail angiogenesis, prevent metastasis and is mediated through inhibition of estrogen and progesterone expression.

Keywords: Breast cancer, MCF-7 cells, methylnitrosourea, Psidium guajava

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