Research Articles

2019  |  Vol: 5(4)  |  Issue: 4 (July- August)  |  https://doi.org/10.31024/ajpp.2019.5.4.12
Quantification of BSA in therapeutic dairy waste enriched by foam fractionation

Prabir Kumar Datta1*, Goutam Mukhopadhayay2, Amitavo Ghosh3, Mrinmoy Nag4

Department of Pharmaceutical Engineering & PharmaceuticsBengal College of Pharmaceutical Sciences and Research, Durgapur-713212, India

*Address for Corresponding Author

Prabir Kumar Datta

Associate Professor, Department of Pharmaceutical Engineering & Pharmaceutics,

Bengal College of Pharmaceutical Sciences and Research, Durgapur-713212, India

Abstract

Objective: In this study, experiments were carried out to enrich medicinal proteins from dilute dairy waste by using anionic surfactant namely sodium dodecyl sulphate (SDS) as collector for separation of surface active proteins by adsorption on bubbles surface using foam fractionation method and quantified one single protein specifically bovine serum albumin( BSA) in enriched foamate by RP-HPLC. Materials and Methods: Dairy waste water collected from industry was processed and foam fractionated to foamate of concentrated proteins by using a 8cm. diameter and 100 cm. long glass column fitted with sintered glass G-3 sparger(15-40 micron porosity) used for distribution of bubbles generated by passing N2 gas. Foamate were analysed spectrophotometrically as well as by RP-HPLC. The method was evaluated through experiments by varying process controlling parameters like pH, initial and ionic concentration of feed, gas flow rate through feed, waste-surfactant mass ratio in feed by taking 1litre of feed for every experiment. Results: The process was optimised at initial concentration (500µg/ml) , gas flow rate (350ml/min) ,ionic concentration(0.1M of NaCL), pH (5.5) and waste–surfactant mass ratio(1.5:1) with highest enrichment ratio (49.09)  , percent recovery (98.18%) in foamate. BSA was quantified by RP-HPLC analysis as 4.9% (w/w) of enriched proteins in foamate at optimised condition. Mass transfer co-efficient was determined 12.68x10-9 mol/cal/cm2/sec at pH 8.5, feed concentration of 600µg/ml and gas flow rate at 350ml/min. Conclusion: The study focused foam fractionation as lucrative unit operation to concentrate thermo labile medicinal proteins as well as eliminate pollutant proteins from dairy waste water.

Keywords: Foam fractionation; sodium dodecyl sulphate; enrichment; BSA; medicinal proteins; RP-HPLC

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