Ladli Kishorea*, Papiya Mitra Mazumderb
aSchool of Pharmacy and Emerging Sciences, Baddi University, Solan, Himachal Pardesh
bDepartment of Pharmaceutical Sciences& Technology, Birla Institute of Technology, Mesra, Ranchi, Jharkhand- 835215, India
*Address for Corresponding Author
School of Pharmacy and Emerging Sciences, Baddi University, Solan, Himachal Pardesh, India
Objective: Many plants are predominantly used to treat diabetes and have been the main source for various synthetic compounds. Literature suggests that not much work has been done using Trigonella foenum-graecum plant extracts to check their efficacy in attenuating diabetic nephropathy. So, the objective of present study is to evaluate the effect of the extracts of plant seeds in attenuating the progression of diabetic nephropathy in animal models. Material and methods: Diabetic nephropathy was induced in male albino rat by diabetogenic diet and streptozotocin (65 mg/kg b.wt.,i.p.). On the 9th week of the induction of diabetic nephropathy in animals, treatment was given for 40 days to attenuate the condition of diabetic nephropathy in induced rats. Animals were treated with methanolic extract of Trigonella foenum-graecum Linn. (METFG) at two dose levels (500 mg/kg and 1 g/kg) and with standard drug Glimipride (2mg/kg) for 40th day in order to analyse its renoprotective effect, which was evaluated by means of metabolic profile, renal function test and electrolyte concentration in blood and urine and renal tissue test with its antioxidant status and histopathological study. Results and conclusion: Nephropathy was noted in diabetic induced rats between 8th-9thweeks as assessed by exhibited statistical significant polyuria and increased in serum glucose, creatinine, blood urea nitrogen levels and albuminuria and proteinuria. Nodular glomerularsclerosis, hyaline arteriolosclerosis with chronicglomerulonephritis(GN) was observed by renal morphological study. On 40 days of treatment of METFG; metabolic profile including the renal functions were greatly improved as evidenced by amelioration of creatinine, blood urea nitrogen, total protein concentration, albumin and serum electrolytes and also improvement in antioxidant with decreased in malondialdehyde and increased in catalase, superoxide dismutase and glutathione-s-transferase levels. It ameliorated renal hypertrophy, nodular glomerularsclerosis, hyaline arteriolosclerosis and GN in diabetic nephropathy rats. These studies suggest that Trigonella foenum-graecum Linn seed possess a protective effect and attenuates the progression of diabetic nephropathy in rat.
Keywords: Trigonella foenum-graecum Linn, Diabetic nephropathy, renal function