Research Articles

2019  |  Vol: 5  |  Issue: Articles in press
High sugar induced changes in gut microflora in Drosophila melanogaster: Protective role of Gymnema sylvestre

Hassan Rangegowda Harshavardhana, Mysore Siddaiah Krishna*

Drosophila Stock Centre, Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore 570006, India

*Address of Corresponding Author

Prof. Mysore Siddaiah Krishna

Drosophila Stock Centre, Department of Studies in Zoology, University of Mysore, Manasagangotri, Mysore 570006 India

Abstract

Objective: Animal nutrition and gut microbiota are co influenced by each other in modulating host physiology. Present investigation has been undertaken to understand the effect of age and high sucrose diet on gut microflora in D. melanogaster and protective role of Gymnema sylvestre (GS) on toxic effects of high sucrose and altered gutmicrobial diversity. Material and methods: Flies of Drosophila melanogaster (w118) was raised in control diet, 30% high sugar diet and treatment diet and further subjected for analysis of   gut microbial diversity. Results and conclusion: It was noticed in D. melanogaster that gutmicrobiota vary with age and high sucrose diet. The investigation revealed that gut microbial flora in relation to host age corresponds to Acetobacter and Lactobacillus species. A. pomorum, A. tropicalis, L. brevis, L. fructivorans, and L. plantarum. The relative abundance of each of the above species varies in relation to host age, Density of L. fructivorans and L. plantarum was found to be lowest in young aged flies and it was increased with increasing fly age and highest density was noticed in old aged flies. Further A. tropicalis and A. pomorum were strongly represented in middle-aged flies.  The present study also revealed that the species of Lactobacillus and Acetobacter showed diversity in relation to dietary sucrose. The density of Lactobacillus brevis and L. plantarum was high in flies fed with 30% sucrose compared to flies fed with control diet. Further flies fed with 30% sucrose along with leaf extract of Gymnema sylvestre have a significant influence on the gut microbiota. Thus these studies clearly suggest that host physiology changes with age and diet, in turn, it has a significant influence on resident gut microbial diversity in D. melanogaster. Further, Gymnema sylvestre feeding had an influence on bringing back to normal gut microbial diversity.

Keywords: Gut microflora, Drosophila melanogasterGymnema sylvestre, high sugar diet

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