Research Articles

2019  |  Vol: 5(1)  |  Issue: 1(January-February)  |  https://doi.org/10.31024/ajpp.2019.5.1.3
Phytochemical screening and antimicrobial activity of wild and tissue cultured plant extracts of Tylophora indica

A. Vanitha, S. Vijayakumar, V. Ranjitha, K. Kalimuthu*

Plant Tissue Culture Division, PG and Research Department of Botany, Government Arts College (Autonomous), Coimbatore-641018, India

*Address for Corresponding Author

K. Kalimuthu

Plant Tissue Culture Division, PG and Research Department of Botany, Government Arts College (Autonomous), Coimbatore-641018, India

Abstract

Objective: To analyze phytochemical of wild and tissue cultured plant ethanol and methanol extracts of Tylophora indica through FTIR, GCMS and evaluation of antimicrobial activity against four human pathogens. Materials and Methods: Preliminary phytochemical screening was done by standard methods. Analysis of the wild and tissue culture plants were carried out through the potassium bromide (KBr) pellet (FTIR grade) method. The GC – MS analysis was carried out using a Clarus 500 Perkin – Elmer (Auto system XL) Gas Chromatograph equipped and coupled to a mass detector. Antimicrobial activity of ethanol and methanol extracts of wild and tissue cultured plant samples in Tylophora indica against bacterial pathogens and also two fungal pathogens by agar well diffusion method. Results: All the extracts of the selected plant was found to contain alkaloids, flavonoids, steroids and triterpenoids. Glycosides and Anthraquinones were present only in wild and tissue cultured plant ethanol extracts not in methanol extracts. The FT-IR spectrum showed the presence of alkyl halides (C-Br), aliphatic amines (C-N), alkyl halides, aromatic amine, alkanes, aromatics, amine and β- saturated esters. GCMS analysis of wild and tissue cultured plant of ethanol, methanol extracts were identified 56, 40,111, 90 compounds with 6, 2, 9, 8 bioactive compounds respectively. The antimicrobial activity of ethanol extracts of wild and tissue cultured plant showed good antibacterial activity at the concentration of 60 μl against Bacillus Subtilis (13 mm) and Klebsiella pneumoniae (10 mm) respectively. Conclusions: The results confirmed the fact that this plant possesses effective bioactive compounds useful for our health. Further isolation and biological screening will be providing the therapeutic potential of the compounds. . In addition, the results revealed that the tissue cultured plant extracts was effective in the same way as wild plant extracts.

Keywords: Antimicrobial, FTIRGCMS, In vitro and Tylophora indica

Manuscript Management System
Submit Article Subscribe Most Popular Articles Join as Reviewer Email Alerts Open Access
Our Another Journal
Another Journal
Call for Paper in Special Issue on

Call for Paper in Special Issue on