Vijaykumar Chavan1*, Tanmay K. Chache1, Vikas Yadav2
1Ramnarain Ruia Autonomous College, L. N. Road, Matunga (East), Mumbai 400 019 India
2Department of Chemistry, Ramnarain Ruia College, Mumbai, Maharashtra, India
*Address for Corresponding Author
Dr. Vijaykumar Chavan
Ramnarain Ruia Autonomous College, L. N. Road, Matunga (East), Mumbai 400 019 India
Objective: A precise, accurate and reproducible HPLC method is validated for simultaneous quantification of three bioactive markers Gallic acid, myricetin and quercetin in the plant of Syzygium cumini and its marketed formulation. Materials and methods: The mobile phase was a gradient mixture of 0.1% OPA and methanol 65:35 (v/v). The column used for the chromatographic technique was Phenomex, C-18 having dimensions of 250 mm x 4.6 mm, 5 μm. The detection of chromatograms was carried out at 220nm. This HPLC method was validated statistically and recovery study was performed to confirm the accuracy of the method. Results and conclusion: A precise method for simultaneous quantification of Gallic acid, myricetin and quercetin was developed by using High performance Liquid Chromatography. The analytical standards were compared that to methanolic extract of plant and formulation with their respective Retention time. The method can be used for routine quality control of herbal raw materials as well as formulations containing any or all of these compounds.
Keywords: Simultaneous quantification, HPLC, gallic acid, myricetin and quercetin