Sachin Shivling Bhusari*, Shivani Gokul Bhokare, Kanchan Diliprao Nikam, Avinash Narayanrao Chaudhary, Pravin Shridhar Wakte
1University Department of Chemical Technology, Dr.Babasaheb Ambedkar Marathwada University, Aurangabad, Maharashtra, India
*Address for Corresponding Author
Dr. Sachin Shivling Bhusari,
Assistant Professor,
Pharmaceutical Technology Division,
Department of Chemical Technology,
Dr. Babasaheb Ambedkar Marathwada University, Aurangabad - 431001, Maharashtra, India
Abstract
Objective: The main objective of the present work is to study the pharmacognostic and phytochemical characteristics of Pergularia daemia stems belonging to the family Asclepiadaceae. Materials and Methods: Ethanolic and aqueous extracts of Pergularia daemia stems were prepared by using Soxhlet and cold maceration technique respectively. The extracts were concentrated using rotary evaporator. Morphological evaluation, microscopical characterization, powder analysis, physicochemical evaluation, fluorescence analysis, preliminary phytochemical screening and TLC profiling of Pergularia daemia was performed. Results: Pharmacognostic and phytochemical studies of stem of Pergularia daemia. Microscopical characters were determined by performing transverse section of stem and powder microscopy. Standardization of whole plant was done with the help of ash value (total ash, acid insoluble ash and water soluble ash), water soluble extractive and alcohol soluble extractive value. Fluorescence analysis was also carried out ultraviolet chamber. Alcoholic and aqueous extracts were prepared and preliminary phytochemical analysis was carried out. Alcoholic extract shows the presence of carbohydrates, alkaloids, flavonoids whereas aqueous extract shows presence of steroids and tannins. Thin layer chromatography of alcoholic extract shows the eight numbers of spot having Rf value 0.12, 0.8, 0.38, 0.45, 0.58, 0.74, 0.87 and 0.93 respectively. Conclusion: Pharmacognostic and phytochemical investigation of powdered stem of Pergularia daemia describing its morphological evaluation, microscopical characterization, powder analysis, physicochemical evaluation, fluorescence analysis, preliminary phytochemical screening and TLC profiling has been studied in detail so as to develop a reference for academic and commercial purpose. Further, it can be used for the standardization and pharmacopoeial parameters development.
Keywords: Pegularia daemia, extraction, morphology, microscopy, phytochemical analysis
