Research Articles

2018  |  Vol: 4(4)  |  Issue: 4 (July- August)  |  https://doi.org/10.31024/ajpp.2018.4.4.13

Phytochemical and Antimicrobial evaluation of Endophytic Alternaria alternata isolated from Terminalia arjuna (Roxb.) Wight & Arn.

Jagadevi Shivaputrappa¹, Vidyasagar G. M.^*

¹Research scholar, Medicinal plants and Microbiology research laboratory, Department of Post Graduate Studies and Research in Botany, Gulbarga University, Gulbarga- 585106, Karnataka, India.

^*Professor, Medicinal plants and Microbiology research laboratory, Department of Post Graduate Studies and Research in Botany, Gulbarga University, Gulbarga- 585106, Karnataka, India.

*Address for Corresponding author

Dr. Vidyasagar G. M.                                               

Professor, Medicinal plants and Microbiology research laboratory, Dept. of PG Studies and Research in Botany, Gulbarga University, Gulbarga - 585 106, Karnataka, India.  

Abstract

Objective: The present study was carried out to investigate the chemical constituents by preliminary tests and the antimicrobial activity of various extracts of an endophytic fungi Alternaria alternata isolated from the stem bark of Terminalia arjuna. Materials and methods: The phytochemical analysis of all the extracts of fungi was carried out by referring the standard procedures and antimicrobial activity was carried out by agar well diffusion method. Results: The phytochemical investigation of all the extracts of fungi showed the presence of alkaloids, phenols, flavonoids, tannins, saponins, steroids, triterpenes and glycosides.  The methanol, ethanol and ethyl acetate extracts of endophytic fungi were tested against four human pathogenic Bacteria like, Klebsiella pneumoniae, Salmonella typhi, Staphylococcus aureus and Escherichia coli and four Candida species  like, C. albicans [MTCC 1637], C. glabrata [MTCC 3019], C. haemulonii [MTCC 1966] and C. tropicalis [MTCC 230] by agar well diffusion method. The methanolic and alcoholic extract of A. alternata at 40mg/ml showed maximum activity against S. aureus (15.6mm) and C. albicans (10.6mm), respectively. Conclusion: Alternaria alternata possess several bioactive molecules which are responsible for antimicrobial potential, may serve as an alternative source for the treatment and control of several microbial infections.

Keywords: Terminalia arjuna, Alternaria alternate, Endophytic fungi, antibacterial, anticandida