Keshav Dhakal1*, Kriti Chaudhary1, Sunita Paudel1, Mamta Pant1, Chandrika Adhikari1, Ramesh Raj Padhaya1 , Pratigya Sapkota2, Ankit Acharya3
1Department of Pharmacy, Asian College for Advance Studies, Satdobato, lalitpur, Nepal
2School of Health and Allied Science, Pokhara University, Kaski, Nepal
3Department of Pharmacy Practice, Sri Adichunchanagiri College of Pharmacy, B.G. Nagara-571448, Karnataka, India
Address for Corresponding Author
Keshav Dhakal
Assistant professor,
Department of Pharmacy,
Asian College for Advance Studies, Satdobato, Lalitpur, Nepal
Abstract
Objective: The aim of this research was to assess the anti-oxidant, anti-epileptic and anti-anxiety properties of the flavonoids obtained from the Pomegranate (Punica granatum L.). Materials and Methods: Methanol and ethyl acetate solvent was used for Soxhlet extraction and water was used for cold maceration and the extracts were screened for the presence of different phytochemicals. Anti-oxidant activity was assessed using DPPH method by using ascorbic acid as standard and IC50 value was calculated. Results and Conclusion: Flavonoid obtained from the methanolic extract showed highest free radical scavenging activity with IC50 value of 31.48µg/ml. Anti- epileptic activity was assessed using the MES model using Phenytoin (25 mg/kg) as standard and MEP and EAP were given at the doses of 100mg/kg and 200 mg/kg in which 200mg/kg methanolic extract showed the least duration of tonic clonic convulsion (26.67 + 0.33 sec). Actophotometer test was done to evaluate the anti-anxiety activity in which Diazepam (3 mg/kg) standard drug was used and MEP and EAF (100mg/kg and 200mg/kg) was administered to the mice. The result showed that the MEF 200mg/kg showed the least movement during the 3 minutes time.
Keywords: Punica grantum, phytochemical screening, anti-epileptic activity, anti-anxiety, antioxidant activity
