Ashish Garg1, Ajay Shukla2, Prakash Pandey3, Suresh Dev2
1Department of Pharmaceutical Science, Guru Ramdas Khalsa Institute of Science & Technology Pharmacy, Jabalpur, India
2Department of Pharmaceutical Science, Mohanlal Sukhadia University, Udaipur Rajasthan, India
3Department of Chemistry and Pharmacy, Rani Durgavati University, Jabalpur, India
*Address for Corresponding Author:
Ashish Garg
Department of Pharmaceutical Science,
Guru Ramdas Khalsa Institute of Science & Technology Pharmacy,
Jabalpur, India
Abstract
Objective: Ociumum tenuiflorum also known to the world as O. sanctum, Holy basil or Tulsi, is an aromatic plant show variety of pharmacological and therapeutical activities. In present study, ethanolic extract of Ocimum sanctum leaves was evaluated for inhibition of calcium oxalate crystals by in vitro methods. Materials and methods: The inhibitory activity of ethonolic extract of Ocimum sanctum leaves was seen to be similar to that of Cystone (a proprietary drug for kidney stone treatment). Fresh plant was collected and it was shade dried and then the leaves were powdered and extracted with alcohol with the help of soxhlet apparatus and the dried leaves were macerated with water for seven days for aqueous extract. The formation of calcium oxalate crystal (frequently found component of most urinary stones) can blocked in-vitro by ethonolic extract of O. sanctum and identified by titrimetric method. Results: Ethanolic extract of leaves of Ocimum sanctum shows effective calcium oxalate crystallization inhibition 77.18±1.15% in vitro, where as cystone, a drug with more potential for renal calculi showed calcium oxalate inhibition (92.34±0.96%) in terms of transformation of calcium oxalate precipitation. Conclusion: The result of current study shows that the ethonolic extract of Ocimum sanctum leaves showed similar activity to that of cystone in process of restricting the formation of calcium oxalate precipitate. The Alcoholic extract of Ocimum sanctum leaves could be further determined in vivo and then characterization of its active constituent could lead to a novel drug for patients with Urolithiasis.
Keywords: Ocimum sanctum. Ethanolic extract, cystone, urolithiasis, calcium oxalate crystal
