Bharathi D.R.1 , Abubaker Siddiq2*, Nikhil K.2, Nataraj G.R.2, Abhinandan M.2
1Department of Pharmacology, Sri Adichunchanagiri College of Pharmacy, Adichunchanagiri University, BG Nagar-571418, Mandya, Karnataka, India
2Department of Pharmacology, SJM College of Pharmacy, Chitradurga- 577502, Karnataka, India
*Address for Corresponding Author
Dr. Abubaker Siddiq
Department of Pharmacology, SJM College of Pharmacy, SJM Campus, Chitradurga, Karnataka, India
Background: The immune system has a fundamental role in protecting the body against pathogenic microbial agents. Environmental pollutants and dietary habits cause disturbances in immune activities and diet containing micronutrients and antioxidants are known to prevent these alterations. Objective: The aim of the present study was to investigate the immunomodulatory potential of ethanolic extract of Annona squamosa leaves and the extract reported to contain polyphenols, flavonoids and tannins. Material and methods: Ethanolic extract of Annona squamosa leaves at the doses of 50 mg/kg and 100 mg/kg (per oral) were studied for the assessment of Immunomodulatory activity. To induce immunosuppression, pyrogallol (100 mg/kg, i.p.) was administered. The mice were sensitized with sheep red blood cells (SRBC) to assess the immunological study. In cellular immune response model, SRBC (0.5x109 cells/100g, i.p.) were injected in the sub plantar region of the hind paw. Results and conclusion: An increase in paw volume was recorded in pyrogallol-induced immunosuppressed mice. The non-specific immune response was assessed by carbon clearance assay. The Leaf extract of Annona squamosa increased the footpad thickness and phagocytic index in carbon clearance assay. From the above findings, it is concluded that ethanolic extract of Annona squamosa leaves possesses potential Immunomodulatory activity.
Keywords: Annona squamosa, Pyrogallol, immunomodulatory, flavonoids