Research Articles

2020  |  Vol: 6(1)  |  Issue: 1 (January-February)  |  https://doi.org/10.31024/ajpp.2020.6.1.7

Evaluation of serum trace elements, lipids profile, ferritin and 8-hydroxydeoxyguansine levels in Yemeni patients with beta thalassemia major

Mansour S. Al-Kobati¹, Hassan M. Al-Mahbashi^1*, Badria Shamsan²,   Najla AL-Sonboli³, Nawfal A.M. Numan⁴

¹Department of Forensic Medicine and Clinical Toxicology, College of Medicine, Sana'a University, Sana'a, Yemen

²Department of Biochemistry, College of Medicine, Sana'a University, Sana'a, Yemen

³Department of Pediatrics, College of Medicine, Sana'a University, Sana'a, Yemen

⁴College of Pharmacy &Medical sciences, Al-Ahliyya Amman University, Amman Jordan

*Address for Corresponding Author

Hassan M. Al-Mahbashi (PhD)

Associated Professor of Pharmacology & Toxicology,

Department of Forensic Medicine and Clinical Toxicology, Faculty of Medicine, Sana’a University, Yemen

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Abstract

Objective: The aims of this study was to evaluate the serum level of some trace elements (Copper, zinc and magnesium), lipid profile and 8-hydroxydeoxyguansine(8-OHdG),ferritin  levels in Yemeni patients with BTM. Materials and Methods: In this comparative cross sectional study, forty BTM blood transfusion dependent patients 4 to 22 years old who attended the Yemeni Society Centers for Thalassemia and Genetic Blood Disorders in Yemen were included. Forty healthy subjects served as control group .The serum level of trace elements (Copper, zinc and magnesium) were performed by humolyzer 3500 using spectrum diagnostic reagent kit. The serum levels of 8-OHdG was measured manually by enzyme linked immunosorbent assay (ELISA) kit. Chemiluminescent Microparticle Immunoassay (CMIA) was used for the quantitative determination of ferritin in human serum and plasma .Serum levels of lipid profile were enzymatically determined using reagent kit. Results: There was a significant elevation in serum level of zinc, 8-OHdG and ferritin in thalassemic patient as compared to controls. Copper and magnesium serum level were significantly decreased in BTM patient as compared to control. In addition total cholesterol (TC), low density lipoprotein(LDL) and high density lipoprotein(HDL) were significantly lowered in BTM patient. Conclusion: The study showed Yemeni patients with BTM have high serum level of 8-OHdG and ferritin which reflect an increase oxidative stress status. Also the results showed low serum level of copper and magnesium, and a high serum level of zinc and dyslipidemia.     

Keywords: Beta Thalassemia Major, Trace Elements, 8-hydroxydeoxyguansine, oxidative stress