Research Article

2016  |  Vol: 2  |  Issue: 1 (Jan- Feb)

Investigations on hydroalcoholic extract of Zizyphus oenoplis for analgesic and anti-nociceptive activity

Ajay Shukla¹, Shweta Garg², Ashish Garg², Pramod Mourya², C.P. Jain¹

¹Department of Pharmaceutical Science, Mohanlal Sukhadia University Udaipur Rajasthan, India

²Department of Pharmacognosy Guru Ram Das Khalsa Institute of Science & Technology Pharmacy, Jabalpur, India

Correspondence address:

 Mr. Ajay Shukla

Department of Pharmaceutical Science, Mohanlal Sukhadia University Udaipur Rajasthan, India

Abstract

Objective: The aim of the present study was to investigate analgesic and anti-nociceptive activity of hydroalcoholic extract of Zizyphus oenopli leaves. Materials and methods: Hydralcoholic extract of Zizyphus oenoplis leaves was tested for phytochemical analysis. Swiss mice were selected for study of analgesic and anti-nociceptive activity of Zizyphus oenoplis    extract at doses 50, 100 and 200 mg/kg p.o. Various models viz. acetic acid induced writhing model, Eddy's hot plate and tail flick model were selected for analgesic study. Another model tail immersion model was used for anti-nociceptive study. Results: Effect of Z. oenoplis extract in acetic acid induced writhing model, found significant at 200 mg/kg dose and comparable to the standard group Diclofenac sodium (25mg/kg). In case of hot plate model, the maximium activity was observed at 90 min at dose of 200 mg/kg, which was less than the reference drug morphine sulfate (1.5 mg/kg i.p.). The analgesic effect on the tail flick model was found comparable with morphine sulfate in dose dependent manner. Anti nociceptive effect of Z. oenoplis extract in formalin-induced paw licking model, was found significantly in the early phase at 100 and 200 mg/kg p.o. and comparable to the Diclofenac sodium (25mg/kg) group. Conclusion: Z. oenoplis found effective in both type non-narcotic and narcotic nociception models which suggesting its possible activity via peripheral and central mechanism. The activity can be concluded due to presence of alkaloids and terpenoids in hydroalcoholic extract of Z. oenoplis.

Keywords: Zizyphus oenoplis, analgesic, acetic acid, Eddy′s hot plate, tail flick