NAGALO Ousmane1*, ROBET Émilie Jocelyne1, KOUASSI Kousso Brigitte2, KONE Mama3
1Training and Research of Unit Science and Technology, Alassane Ouattara University, 01 BO box V18 Bouaké, Côte d’Ivoire.
2Natural Sciences Training and Research Unit, Nangui Abrogoua University, Laboratory of Physiology, Pharmacology and Pharmacopoeia, 02 BO box 801 Abidjan 02, Côte d’Ivoire.
3Training and Research of Natural Sciences, Laboratory of Physiology, Pharmacology and Pharmacopoeia, Nangui Abrogoua University,02 BO box 801 Abidjan 02, Côte d’Ivoire.
*Address for Corresponding author
Dr NAGALO Ousmane
Assistant, Training and Research of Unit Science and Technology, Alassane Ouattara University, Bouaké, Côte d’Ivoire.
Abstract
Sacoglottis gabonensis is a plant used in the traditional treatment of infectious diseases such as Buruli ulcer and leprosy in Côte d'Ivoire, which lasts an average of six months. The objective of this study was to verify the safety of the total aqueous extract of Sacoglottis gabonensis stem bark (TAESg) in Wistar rats over a six-month period. 100 rats were divided into six groups, namely A, B, C and D of 20 rats/group and E, F of 10 rats/group. Groups A and E received distilled water ; groups B and C received TAESg at doses of 3.5 and 17.5 mg/kg bw; groups D and F received 35 mg/kg bw orally at a rate of 1 mL/100 g bw. Groups E and F were used to assess the persistence, reversibility or appearance of delayed toxic effects. The animals were weighed at the beginning and then weekly during the experiment. The quantities of food consumed were measured daily. At the end of the experiment, venous blood taken from the retro-orbital sinus was collected in EDTA tubes to determine the chronic effect of TAESg on hematological parameters. This study showed that daily oral administration of TAESg for six months had no effect on clinical signs of toxicity. No significant variation in feed intake and weight gain was observed. The erythrocyte count and erythrocyte indices ; the leukocyte count and leukocyte indices did not change. However, only the MCHC increased very highly in groups C and D. As for the average platelet count, it increased significantly in group D. Ultimately, TAESg had no effect on clinical signs of toxicity or anthropometric parameters, and was nontoxic on hematological parameters for six months. It is also necessary to evaluate the chronic effect of this extract on clinical biochemistry in rats.
Keywords: Sacoglottis gabonensis, safety, anthropometric, hematological parameters
