Research Articles

2021  |  Vol: 7(5)  |  Issue: 5(September- October)  |  https://doi.org/10.31024/ajpp.2021.7.5.4
Preliminary phytochemical screening, quantitative estimation and in-vitro antioxidant activity of Anamirta cocculus (L) fruits

Prakash Dabadi1*, Chandrashekhar Venkaraddi Mangannavar2, Mallappa Shalavadi2

1Department of Pharmacology, Bapuji Pharmacy College, S.S. Layout, Davanagere-577004,  Karnataka, India

2Department of Pharmacology, BVVS's Hanagal Shri Kumareshwar College of Pharmacy, Bagalkot-587101, Karnataka, India

*Address for Corresponding Author:

Mr. Prakash Dabadi*

Assistant Professor

Department of Pharmacology,

Bapuji Pharmacy College, Davangere-577004, Karnataka, India

Abstract

Objective: Plants are important sources of biological activities natural yields, the present study was considered to evaluate the physicochemical, preliminary phytochemical, quantitative and antioxidant activity of hydroalcohol extract of Anamirta cocculus fruits (HEAC). Material and methods: The Physicochemical properties were carried out from crude powder, such as total ash, acid insoluble ash, water-soluble ash, extractive values and loss on drying (LOD). The HEAC fruits was subjected to phytochemical investigation. Quantitative estimation of total phenolic and flavonoids content was estimated by Folin ciocalteu and aluminium chloride by colorimetric method. In vitro antioxidant were analysed by using DPPH assay, hydroxyl radical scavenging, and lipid peroxidation activity. Results: Phytochemical analysis exposes the accessibility of ancillary various metabolites are alkaloids, phytosteroids, glycosides, flavonoids, tannins, saponins and phenolic compounds in the extract. The quantitative analysis for HEAC fruits was done for total phenolic content (TPC), total flavonoids content (TFC) were found to be 16.16 ± 0.085 mg of Gallic acid(GA) and 299.9 ± 24.63mg of Quercetin equivalent wt/ g of extract respectively. The extract was showed powerful hydroxyl radical scavenging and lipid peroxidation activity (IC50 values of 364.78µg/ml, 124.40µg/ml, 358.09µg/ml) respectively. The current study revealed that HEAC fruits have significant free radical scavenging activity. Conclusion: Based on our outcomes, it was initiated HEAC fruits can act as a source of a beneficial drug due to presence of various active phytoconstituents, and extract showed potential antioxidant activity. These properties further act as supportive for preventing several diseases triggered by oxidative stress.

Keywords: Anamirta cocculus, hydroalcohol, phytochemicals, antioxidant, flavonoids

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