Research Article

2015  |  Vol: 1(1)  |  Issue: 1(Sept- Oct)
Isolation of embelin from Embelia ribes and evaluation of its anti-Cancer potential in breast cancer

Veerpal Kaur,1 Supandeep Singh Hallan,1 Nidhi,1 A.N. Kalia,2 Neeraj Mishra1*

1Department of Pharmaceutics, ISF College of Pharmacy, Moga, Punjab, India

2Department of Pharmacognosy, ISF College of Pharmacy, Moga, Punjab, India

*Corresponding Author

 Dr. Neeraj Mishra

Department of Pharmaceutics, ISF College of Pharmacy,

 Ferozepur Road, Ghal Kalan

 Moga, and Punjab, India Postal Code: 142001,

 Tel Nos. 01636-324200, Fax No. 01636-239515

 Email: neerajdops@rediffmail.com

Abstract

Objective: Aim of present study to evaluate anticancer activity of dried fruit of Embelia ribes containing embelin in various types of cancer. Material and Method: Embelin was extracted and isolated from Embelia ribes berries of Indian origin. Isolated embelin was characterized by melting point, TLC, partition coefficient, solubility, FT-IR, NMR and Mass spectroscopy. Assessment of anticancer activity was done by Cytotoxicity assay and Apoptosis assay by using MCF‑7 breast cancer cells. Isolated Embelin was investigated on MCF-7 breast cancer cell line by MTT assay and apoptosis assay. Different concentration of embelin were used and observed that 80μg/ml was the IC50 value of embelin in MCF‑7 breast cancer cells and it was found that embelin was able to affect the viability of cells and also induce apoptosis of MCF‑7 breast cancer cells in a dose dependent manner. Results: We have found that the isolated embelin have significant (٭p < 0.0001) potency against the MCF-7 breast cancer cells in dose dependent manner and showed significant increase in apoptotic cells at IC50 value. Conclusion: Isolated embelin have significant potency against the MCF-7 breast cancer cells in dose dependent manner.

Keywords: Embelin, Apoptosis, Cancer, Cytotoxicity, Breast Cancer, TLC.

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