Ravi Ahirwar, Kajal Khan, Surendra K. Jain, Rajesh Singh Pawar*
Truba Institute of Pharmacy, Karond Bypass Rd, Gandhi Nagar, Bhopal, Madhya Pradesh 462038 Bhopal
*Address for Corresponding Author
Dr. Rajesh Singh Pawar
Professor and Principal
Truba Institute of Pharmacy, Karond Bypass Rd, Gandhi Nagar, Bhopal, Madhya Pradesh 462038 India
Abstract
Objectives: To evaluate the antioxidant, antidiabetic effect of the polyherbal formulation of methanolic extract of Trigonella foenum-graceum (seed) and Holarrhena pubescens (stem) on alloxan induced type-2 diabetes in rats. Materials and Method: Rats were divided into five groups (n=6). Group-I (Normal control) received normal saline. Group-II (Negative control) received 120 mg/kg of Alloxan, treated subsequently with normal saline.Group-III and IV (Test group Test-1&2) received 250, 500 mg/kg bw of TFG+HP methanolic extract. Group-V (Standard) received Glibenclamide (3mg/kg p.o). The qualitative analysis of various phytochemical constituents and quantitative analysis were determined. Theantidiabetic effects studied using in vivo glucose uptake in an isolated alloxan-induced diabetic rat model. The in vitro anti-oxidant activity of extracts of Trigonella foenum-graecum and Holarrhena pubescens was evaluated by DPPH radical scavenging activity. Results: Their phytochemical analysis revealed variable phytoconstituents in the methanolic extracts. In vitro antioxidant activity was measured using the one, DPPH assay, with methanolic extracts from Trigonella foenum-graecum and Holarrhena pubescens showing IC50 values of 37.05 and 32.32, respectively, close to the standard (Ascorbic acid). The test samples showed a significant (P<0.05) reduction in blood glucose levels compared to the alloxan-treated group. The body weights for the normal control, alloxan-treated, and polyherbal formulation-treated groups (at doses of 250 mg and 500 mg) were recorded. Conclusion: The results obtained in present study clearly demonstrate that the methanolic extracts from Trigonella foenum-graecum seeds and H. pubescens stems exhibited significant antioxidant activity and moderate antidiabetic activity, suggesting the need for further exploration in animal models and the isolation of active constituents.
Keywords: Trigonella foenum-graecum, Holarrhena pubescens, alloxan-induced, phytochemical, antidiabetic, polyherbal
