Zaynat S. Khashimova, Komola A. Kakhorova*, Malika K. Salakhutdinova, Muxabbatxon O. Kalanova, Yuliya I. Oshchepkova, Shavkat I. Salikhov, Zaynat Sattarovna Khashimova
A. S. Sadykov Institute of Bioorganic Chemistry, Academy of Sciences of the Republic of Uzbekistan, Tashkent, 700125, Uzbekistan, 100125
*Address for Corresponding Author
Komola Azamatovna Kakhorova
Doctor of Philosophy,
A. S. Sadykov Institute of Bioorganic Chemistry,
Academy of Sciences of the Republic of Uzbekistan, Tashkent, 700125, Uzbekistan, 100125
Abstract
Lectins are one of the functionally significant proteins involved in the plant protective strategy and possess a wide range of action, which in many ways stimulates researchers to search for new sources of their isolation. Objective: The work was initiated to study cytotoxic activities and membrane-active properties of lectin-like glycoproteins isolated from the dodder (Cuscuta europaea). Methodology: LLP were isolated from the seeds of the dodder by extraction with phosphate buffer solution with subsequent gradient precipitation with 20%, 50% and 80% ammonium sulfate and designated as LLP20, LLP50 and LLP80, respectively. Cytotoxic activity of LLP was assessed biochemically in HeLa (cervical carcinoma) and B-16 (skin cancer) cell cultures by MTT, LDH assay. The membrane-active properties of the total fraction of dodder containing LLP were studied, as well as its effect on the conductivity of the lipid bilayer. Results: The results showed that the B-16 cell line was more sensitive than HeLa to lectin-like proteins of Cuscuta europaea. Also, it was shown that the HeLa cell line was more resistant than B-16 to cisplatin. All fractions contribute to the manifestation of high activity of extracellular LDH. Conclusion: Thus, we isolated and characterized lectin-like proteins form Cuscuta europaea, as well as demonstrated cytotoxic effect of LLP20, LLP50 and LLP80 on HeLa and B-16 cells. The membrane-active properties of LLP, as well as heir influence on the conductibility of the lipid bilayer and the degree of cell membrane damage, are shown.
Keywords: Lectin-like proteins, Cuscuta europaea, cytotoxicity, membrane –active properties, cisplatin