Sharad V. Bhandarkar, Deepak S. Mohale, Anil V. Chandewar, Nitin I. Kochar
P. Wadhwani College of Pharmacy, Yawatal, Maharshtra, India
*Address for Corresponding author
Mr. Sharad V. Bhandarkar
P. Wadhwani College of Pharmacy, Yawatal, Maharshtra, India
Abstract
This study explores the phytochemical composition, Pseudevernia furfuracea (L) Zopf. thin-layer chromatography (TLC) profiles, and antioxidant activity of acetone. Phytochemical analysis reveals that acetone is the most versatile solvent, extracting a wide range of compounds including alkaloids, saponins, glycosides, flavonoids, triterpenes, and steroids. Methanol also extracts multiple phytochemicals but is less comprehensive than acetone. Petroleum ether predominantly extracts alkaloids and glycosides, while chloroform selectively extracts proteins and tannins. Carbohydrates were not detected in any of the extracts. TLC analysis indicates that acetone extracts show the highest mobility of compounds with an Rf value of 0.64, reflecting a strong affinity for the mobile phase. Methanol extracts have the highest Rf value of 0.78, demonstrating the greatest mobility, whereas petroleum ether and chloroform extracts exhibit lower Rf values of 0.54 and 0.71, respectively. Antioxidant activity By DPPH method
Keywords: Pseudevernia furfuracea, phytochemical analysis, thin-layer chromatography, antioxidant, lichen