Okassa Poma Chancelia Inès1, Peneme Bonaventure Max Lazare1,2, Wossolo Lingomo Berthrand Stephane1,2, Bafounguila-Ngala Syntyche Maïna1, Diamesso-Zola Kwa Nzambi Seigneur Gens1,, Etou Ossibi Arnaud Wilfrid1,2
1Laboratory of Pharmacodynamics and Experimental Pathophysiology, Marien NGOUABI University
2Laboratory of Biochemistry and Pharmacology, Faculty of Sciences and Health, Marien NGOUABI University
Abstract
The objective of this work was to evaluate the estrogenic effect of the aqueous extract of the roots of Thonningia sanguinea in female wistar rats. The acute toxicity study was carried out according to OECD, 2022. Two batches of three mice each received respectively distilled water and the aqueous extract of the roots and Thonningia sanguinea at a single dose of 5000 mg/kg, then weight monitoring as well as food consumption and water intake were noted for a duration of fourteen (14) days. We see that this extract is well tolerated in mice with an LD50 > 5000 mg/kg. To investigate the estrogenic effects of this plant, the animals were treated for 28 days with distilled water and the aqueous extract of the roots of Thonningia sanguinea at doses of 200 and 400 mg/kg. At a dose of 200 mg/kg, this extract causes an increase in the weight of the uterus, ovaries and adrenal glands, as well as an increase in estrogen and progesterone levels. In conclusion, chemical screening revealed the presence of tannins, alkaloids, flavonoids, free anthraquinones and saponosides. The estrogenic properties observed are due to the presence of these chemical families found in this extract.
Keywords: Estrogenic activity, Thonningia sanguinea, acute toxicity, anthraquinones, saponosides
