Research Article

2016  |  Vol: 2  |  Issue: 1 (Jan- Feb)

Zero order and first order derivative Spectrophotometric determination of Metformin HCL in bulk dosage form

Rajesh Shukla¹, Sunil Singh²*, Jay Ram Patel², Sarita Kare², Reenu Yadav²

¹Guru Ramdas Khalsa Institute of Science and technology, Barela, Jabalpur, Madhya Pradesh, India

²*Department of Pharmaceutical Chemistry, Oriental College of Pharmacy, Raisen Road Bhopal, Madhya Pradesh, India

*Corresponding author:

Sunil Singh

Oriental College of Pharmacy, Thakral Nagar, Raisen Road,

Bhopal, Madhya Pradesh, India 462021.

Abstract

Objective: A simple, accurate, sensitive and precise Zero order and First order Ultraviolet spectrophotometric method has been developed for the determination of Metformine HCL in bulk dosage form. Materials and methods: The solutions of standard and sample were prepared in methanol. In the UV specrophotometric method, the quantitative determination of the drug was carried for zero order at 234nm and first order at 246nm, the linearity range was found to be 2-12 μg/ml and 2-12 μg/ml respectively. Results: The calibration graphs constructed at their wavelength of determination were found to be linear for zero and first derivative spectrophotometric methods. The proposed methods have been extensively validated statistically that included parameters such as linearity, accuracy, precision, LOD, LOQ, recovery and robustness. There was no significant difference between the performance of the proposed method regarding the mean values and standard deviations. Conclusion: The described methods can be readily utilized for analysis of pharmaceutical formulation.

Keywords: Method development; Validation; Derivative Spectroscopy; Metformin HCL