Pushpendra K. Jain1, Alok Pal Jain2
1Deapartment of Pharmaceutical Sciences, Dr. H. S. Gour University (M.P.), India
2RKDF College of Pharmecy, SRK University, Hoshangabad Road, Jat Khedi, Misrod, Bhopal (M.P.) India
Corresponding Address
Dr. Alok Pal Jain
RKDF College of Pharmecy
SRK University, Hoshangabad Road, Jat Khedi, Misrod, Bhopal
Abstract
Objective: Lichens are symbiotic organisms consisting of a fungus and a photosynthetic partner which can be either an alga or cyanobacterium. The objective of present study was to test dermal wound healing effect of Parmotrema reticulatum lichens with possible antioxidant and antibacterial mechanism. Materials and methods: The acetone extract was separate from powdered P. reticulatum lichens for phytochemical and wound healing screening. In-vivo wound healing activity was investigated using incision and dead space wound methods. Tensile strength and biochemical parameters (collagen and protein content) were determined from healed tissues by standard methods. Results: The results of phytochemical studies showed that acetone extract of P. reticulatum confirmed the presence of flavonoids, glycosides and terpenoids. The tensile strength of skint tissues treated with acetone extract was increased significantly in comparison to control group. The hydroxyproline content and protein content found increased on 9th day after treatment with 5% w/w ointment containing acetone extract. The antioxidant status of healed skin tissues was improved upon treatment with acetone extract ointment (5%w/w). Conclusions: The results of present studies can be concluded that the significant dermal wound healing activity may be due to presence of flavanoids and usnic acid in acetone extract by possible antioxidant and antibacterial activity.
Keywords: Antibacterial, Parmotrema reticulatum, dermal wound healing, usnic acid
