Eric Martial Deutchoua Ngounou*, Faustin Dongmo, Yannick Dimitry Mang, Slestin Sokeng Dongmo, Nicolas Njintang Yanou
Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Cameroon
*Address for Corresponding Author
Eric Martial Deutchoua Ngounou
Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Cameroon
Abstract
Objective: Clerodendrum thomsoniae is an important underutilized plant with established lipid-lowering, anti-free radicle, hypoglycemic and antidiabétic effect. The present study was aimed at evaluating the acute and subchronic toxicity in experimental animals. Materials and methods: Experiments were conducted according to OCDE425 and OCDE407 guidelines for the study of acute and subchronic toxicity. Acute toxicity studies used female and male mices Mus musculus by single dose oral administration of aqueous extract of C. thomsoniae (AECT) at doses of 2000, 5000 and 9000 mg/kg. Subchronic toxicity study was carried out during 28 days by daily oral administration of single dose of AECT on albino rats (male and female). The control group received vehicle (10 mL/kg b.m.) while treated groups (II, III and IV) were administrated AECT at the respective doses of 312.5, 625 and 1250 mg/kg. Results: A single dose oral administration of aqueous extract of C. thomsoniae till 9000 mg/kg did not lead to death and changing in the behavior 14 days observation after administration. However, slight change was observed concerning motility 3hours after oral administration of extract dose more than 6000 mg/kg. Daily administration for 28 days of the extract at the therapeutic dose of 1250 mg/kg did not bring any significant changes on the relative mass of organs, transaminases activities and creatinine level. Conclusion: The study showed that with an LD50 greater than 9000 mg/kg and a potential hepatoprotective effect, the aqueous extract of C. thomsoniae leaves is favorable to the production of an improved traditional medicine, after preclinical and clinical tests.
Keywords: Clerodendrum thomsoniae Linn, leaves, acute toxicity, subchronic toxicity
